Which of the following occurs during hydrolysis of a polypeptide?
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Which of the following occurs during hydrolysis of a polypeptide?
A linear 80-amino-acid polypeptide is hydrolysed into five peptide fragments. Which bond counts are correct?
An enzyme was incubated for 20 minutes, then returned to pH 7 and 25 °C. Which inference is supported?
| Incubation pH | Incubation temperature / °C | Activity during final minute / % | Activity after return / % |
|---|---|---|---|
| 7 | 25 | 100 | 100 |
| 2 | 25 | 18 | 94 |
| 7 | 80 | 8 | 12 |
| 2 | 80 | 2 | 5 |
…
A meal contains 20 g lentil protein and 10 g grain protein. Which conclusion follows?
| Essential amino acid | Lentil / mg g⁻¹ protein | Grain / mg g⁻¹ protein | Minimum / mg g⁻¹ protein |
|---|---|---|---|
| Lysine | 70 | 25 | 45 |
| Methionine | 15 | 35 | 22 |
…
Only folded protease acts. Which preparation yields the most free amino acids after complete digestion at 30°C, and how much water would joining its remaining peptides into one linear chain release?
Peptide bonds are formed between amino acids
Peptide bonds are broken using water
Water molecules are released
New proteins are created from monomers
4 bonds hydrolysed; 76 remain from 80 initial bonds including terminal bonds
5 bonds hydrolysed; 74 remain from 79 initial bonds
5 bonds hydrolysed; 75 remain from 80 initial bonds
4 bonds hydrolysed; 75 remain from 79 initial bonds
Protease 50°C, substrate 70°C; two water molecules.
Protease 70°C, substrate 50°C; one water molecule.
Protease 50°C, substrate 30°C; one water molecule.
Protease 50°C, substrate 70°C; one water molecule.