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    Question
    SLPaper 2
    1.

    Explain how model making helped Watson and Crick to establish the structure of DN

    [2]
    Verified
    Solution

    a. They decided to combine what was known about the chemical content of DNA with information from X-ray diffraction studies 1 mark

    b. They built scale models of the components of DNA 1 mark

    c. Then they attempted to fit them together in a way that agreed with the data from separate sources 1 mark

    d. They made several arrangements of the scale model until they found the best one that fitted all the data 1 mark

    2.

    Distinguish between the chromosomes of eukaryotic cells and prokaryotic cells.

    [1]
    Verified
    Solution

    a. Chromosomes are associated with «histone» proteins in eukaryotes but not in prokaryotes 1 mark

    b. Chromosomes are linear in eukaryotes but circular in prokaryotes 1 mark

    c. Chromosomes are located in the cytoplasm in prokaryotes, but within the nucleus in eukaryotes 1 mark

    3.

    Outline the role of the enzyme helicase in replication.

    [1]
    Verified
    Solution

    Unwinds/separates strands/double helix (by breaking hydrogen bonds) 1 mark

    4.

    Outline the role of the enzyme DNA polymerase in replication.

    [2]
    Verified
    Solution

    a. links nucleotides together to form a new strand of DNA 1 mark

    b. uses pre-existing strand of DNA as template 1 mark

    c. makes covalent bonds between nucleotides 1 mark

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