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    Peptidase enzyme in the digestive system hydrolyses peptide bonds.

    Question
    SLPaper 3

    Peptidase enzyme in the digestive system hydrolyses peptide bonds.

    A tripeptide Ala-Asp-Lys was hydrolysed and electrophoresis of the mixture of theamino acids was carried out at a pH of 6.0. Refer to section 33 of the data booklet.

    1.

    The solubility of a vitamin depends on its structure.

    Identify the vitamin given in section 35 of the data booklet that is the most soluble in water.

    [1]
    Verified
    Solution

    ascorbic acid/vitamin C

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    2.

    Identify the type of metabolic process that occurs in the hydrolysis of the peptideduring digestion.

    [1]
    Verified
    Solution

    catabolism/catabolic

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    3.

    Identify the name of the amino acid that does not move under the influence of theapplied voltage.

    [1]
    Verified
    Solution

    alanine

    Do not accept ala.

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    4.

    Pollution from heavy metal ions has become a health concern.

    Outline how the presence of heavy metal ions decreases the action of enzymes.

    [1]
    Verified
    Solution

    react/bind/chelate with enzyme
    OR
    disrupt ionic salt bridges
    OR
    affect shape of tertiary/quaternary structures
    OR
    precipitate enzymes
    OR
    break/disrupt disulfide bridges/bonds

    Do not accept “changes shape of activesite” by itself.

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    5.

    Outline how lead ions could be removed from an individual suffering from lead poisoning.

    [1]
    Verified
    Solution

    «use of» host-guest chemistry
    OR
    chelation «therapy»

    Accept specific medication/chelatingagent such as EDTA, CaNa2 EDTA, succimer, D-penicillamine, dimercaprol.

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