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    A section of mRNA contains the start codon for translation of a polypeptide by the ribosomes. 5' G C A U G C C A A C U A C C U C C A A C U C C C U C A C A U 3'

    Question
    SLPaper 3

    A section of mRNA contains the start codon for translation of a polypeptide by the ribosomes. 5' G C A U G C C A A C U A C C U C C A A C U C C C U C A C A U 3'

    1.

    Identify the nucleotides of the start codon for the polypeptide.

    [1]
    Verified
    Solution

    AUG ✔

    2.

    State the chemical difference between the 5' end and the 3' end of a DNA strand.

    [1]
    Verified
    Solution

    5' end contains a phosphate group «on carbon 5» OR 3' carbon contains a hydroxyl/OH group «on carbon 3»

    3.

    Within the base sequence shown in the diagram, the sequence for the stop codon UGA appears. Explain the reasons for translation continuing beyond this point in the mRNA.

    [2]
    Verified
    Solution

    a. the gene code occurs in triplets ✔ b. the stop codon would not be part of the reading frame ✔ c. the code for a polypeptide would contain more codons ✔

    4.

    Describe how bioinformatics can help identify genes within the DNA of an organism.

    [2]
    Verified
    Solution

    a. bioinformatics is the application of computer technology to biological information OR information is stored in a database OR bioinformatics is used in analyzing genomes ✔ b. allows for searches/comparisons/analysis of ORF/open reading frame ✔ c. the computer searches for a start codon and a stop codon «and within a distance of 60+ codons» ✔

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