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Active site
The active site is a small region on an enzyme where the substrate binds. It is formed by a specific sequence of amino acids within the enzyme's overall three-dimensional structure.
Denaturation
Denaturation is the process by which the three-dimensional structure of an enzyme is altered, leading to the loss of its function.
Substrate
The molecule that binds to the active site of an enzyme and is converted into product(s) during the reaction.