What Exactly Is Osmosis?
- Osmosis is the diffusion of water, it follows the same principles, but only water molecules move.
- So similarly, water molecules move from dilute to concentrated solutions.
- Or in other words, from areas of low to high solute concentrations.
- Dilute solution = lots of water molecules, few solute particles (high water concentration).
- Concentrated solution = fewer water molecules, lots of solute particles (low water concentration).
Osmosis
The net movement of water molecules from an area of higher water concentration (dilute solution) to an area of lower water concentration (concentrated solution), across a partially permeable membrane.
Why Does Water Move but Not Solutes?
- The membrane controls what passes through:
- Water molecules are tiny (about 0.3 nanometers), easily fitting through membrane pores.
- Solute particles are much larger (glucose is 1 nanometer, proteins are even bigger), making them too big to fit through the pores.
- The membrane prevents solute from moving:
- Solute particles would naturally diffuse to spread out evenly.
- But the membrane blocks them.
- Only water can move to balance things out.
- Diffusion and osmosis happen simultaneously, but osmosis specifically describes what happens when there's a partially permeable membrane that blocks solute but allows water through.
- Without a membrane (normal diffusion):
- Both solute particles AND water would spread out evenly.
- Everything diffuses until equilibrium.
- With a partially permeable membrane (osmosis):
- Solute particles are physically blocked by the membrane - they're too large to fit through the pores.
- Water molecules are small enough to pass through.
- Since solute can't move to balance concentrations, water moves instead.
- Water moves from high water concentration to low water concentration.
- The membrane doesn't actively block anything, it's passive.
- Large molecules physically cannot fit through the tiny pores in the membrane structure.
- It's like how a sieve works
What Happens to Cells in Salty or Sugary Environments?
The effect of osmosis on cells depends on the surrounding solution:
Cells in hypertonic solutions (concentrated)
Hypertonic solution
A solution with lower water concentration than the cell contents (more concentrated). Water moves out of the cell by osmosis.
- Animal cells:
- Cell shrinks and shrivels (crenation).
- Cell membrane wrinkles.
- Cell may become damaged or die.
- Plant cells:
- Cell membrane pulls away from cell wall (plasmolysis).
- Vacuole shrinks dramatically.
- Cell becomes limp.
- Plant wilts.
If you put red blood cells in pure water (very hypotonic), water will rush in by osmosis and cause the cells to swell rapidly and burst within seconds.
Cells in isotonic solutions (same concentration)
Isotonic solution
A solution with the same water concentration as the cell contents. No net water movement occurs.
- Animal cells:
- Cell remains normal size.
- This is the ideal condition for animal cells.
- Plant cells:
- Cell is slightly deflated (flaccid).
- Not fully turgid but not wilted.
- Still healthy but not at maximum firmness.
- Blood plasma is isotonic with red blood cells.
- Water moves in and out of cells at equal rates, maintaining cell size and shape.
- This is why doctors use isotonic saline solution (0.9% salt) for IV drips.
Cells in hypotonic solutions (dilute)
Hypotonic solution
A solution with higher water concentration than the cell contents (more dilute). Water moves into the cell by osmosis.
- Animal cells:
- Cell swells up as water enters.
- Cell membrane stretches.
- If too much water enters, the cell bursts (lysis).
- Animal cells have no cell wall for protection.
- Plant cells:
- Cell swells up as water enters.
- Vacuole expands and pushes outward.
- Cell wall prevents bursting - it's rigid.
- Cell becomes turgid (firm and swollen).
- Covering meat with salt creates a very hypertonic environment.
- Water moves out of bacterial cells by osmosis, causing bacteria to shrivel and die.
- This is how salt preserves food.
- Recognize it's the cell wall that prevents plant cells from bursting even with filled with water.
- That's why they just become turgid (firm).
Why Do Plants Go Limp When Dehydrated?
- Plant cells rely on water pressure for support:
- When full of water, the vacuole pushes against the cell wall.
- This creates turgor pressure, which is what keeps cells firm and rigid.
- Millions of turgid cells keep the whole plant upright.
- Dehydration reduces turgor pressure:
- When water is scarce, soil becomes hypertonic.
- As water moves out of root cells by osmosis, this causes vacuoles to shrink, reducing pressure on cell walls.
- The plant wilts:
- Without turgor pressure, cells can't maintain their shape.
- Leaves therefore start to droop downward, stems will bend or collapse, and the whole plant goes limp.
- Severe dehydration causes plasmolysis:
- As the cell membrane pulls completely away from cell wall, a large gap forms between the membrane and wall.
- Understand that if a lack of water explains all the above, the addition of water fixes it.
- With watering:
- Soil becomes hypotonic again.
- Water enters root cells by osmosis.
- Vacuoles expand, restoring turgor pressure.
- Plant becomes firm and upright again.
- This is why it's possible to save your wilted house plants.
- Do not assume the cell wall provides all the support.
- The cell wall only prevents bursting.
- It's the water pressure (turgor) inside pushing against the wall that makes cells rigid.
- Without water pressure, even with a cell wall, the plant goes limp.
- When explaining why plants wilt, follow this sequence:
- (1) soil becomes hypertonic
- (2) water moves out by osmosis
- (3) vacuole shrinks
- (4) turgor pressure decreases
- (5) cells become flaccid
- (6) plant wilts.
- What is the difference between osmosis and diffusion?
- What happens to an animal cell placed in pure water? Explain why.
- What is turgor pressure and why is it important for plants?
- Explain why salting vegetables makes them release water.
- What is the difference between a turgid cell and a plasmolyzed cell?