Temperature, Density, and Stratification in Water
Stratification
Stratification is a common phenomenon in aquatic ecosystems, where water forms distinct layers due to differences in temperature and density.
- In most aquatic ecosystems, temperature decreases with depth, resulting in warmer, less dense water above and colder, denser water below.
- This layering creates stratification, the formation of distinct layers that resist vertical mixing.
- Stratification affects the distribution of oxygen, nutrients, and organisms, influencing the productivity and stability of aquatic ecosystems.
Relationship Between Temperature and Density
- Water behaves uniquely compared to most substances because it reaches maximum density at 4°C.
- Above or below this temperature, water becomes less dense.
- As a result:
- At temperatures above 4°C, heating decreases density, causing warm water to float.
- At temperatures below 4°C, cooling decreases density, allowing the colder water to float above denser water.
- When the air temperature drops below 0°C, the surface water freezes while deeper water remains liquid at about 4°C.
Thermal Layers in Deep Lakes
1. Epilimnion
- Warm, well-mixed surface layer exposed to sunlight.
- Rich in oxygen (due to air exchange and photosynthesis).
- Supports phytoplankton and aquatic vegetation.
2. Metalimnion (Thermocline)
- Transition zone with rapid temperature decrease.
- Acts as a barrier to nutrient and gas exchange.
- Limits mixing of oxygen and nutrients between upper and lower layers.
3. Hypolimnion
- Cold, dark, and dense bottom layer.
- Poor in oxygen but rich in nutrients from decomposition.
- Decomposition and respiration dominate; photosynthesis is absent.
In Lake Geneva (Switzerland), the thermocline typically occurs between 10-30 m depth, with the hypolimnion remaining at ~4°C year-round.
Seasonal Stratification in Temperate Lakes
Seasonal Stratification in Lakes
Spring Turnover
- Surface and deep waters are both near 4°C and of similar density.
- Wind action mixes the entire water column, redistributing oxygen and nutrients evenly.
Summer Stratification
- The epilimnion (surface layer) warms and becomes less dense.
- Beneath it lies the metalimnion (thermocline), where temperature drops rapidly with depth.
- The hypolimnion remains cold, dense, and isolated from the surface.
- Stratification prevents nutrient mixing, causing surface nutrient depletion and oxygen depletion below.
Autumn Turnover
- Cooling surface temperatures restore uniform density.
- Wind mixing reoxygenates the deeper layers, replenishing nutrient availability.
Winter Inversion
- Surface water cools below 4°C and becomes less dense, forming ice at the surface.
- Beneath, water remains at ~4°C, allowing freshwater ecosystems to survive under ice.
In northern lakes such as Lake Superior, ice forms on the surface while fish remain active below in the stable 4°C water.
Ecological Importance of Surface Ice
- Ice acts as an insulating layer, preventing the complete freezing of water bodies.
- Beneath the ice, water remains around 4°C, allowing fish, amphibians, and microorganisms to survive.
- This insulation maintains oxygen exchange and metabolic activity at minimal levels.
- This property is unique to water.
- If ice were denser than liquid water, entire freshwater ecosystems would freeze solid each winter.
Stratification and Thermoclines in Lakes and Oceans
Thermocline
A thermocline is a layer in a body of water where temperature decreases sharply with depth, restricting vertical movement and mixing.
- A thermocline is a distinct layer where water temperature drops rapidly with depth, separating the warm, mixed surface layer (epilimnion) from the cold, dense deep layer (hypolimnion).


