IB Biology Topic D1.3.9 Use of the CRISPR Sequences and the Enzyme Cas9 in Gene Editing Revision Notes (HL)

IB Biology Topic D1.3.9 Use of the CRISPR Sequences and the Enzyme Cas9 in Gene Editing Revision Notes (HL) | RevisionDojo

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CRISPR-Cas9 Edits DNA At A Chosen Site

CRISPR-Cas9 is a gene-editing tool adapted from a bacterial defense system.
Unlike a random mutation, it targets a specific DNA sequence chosen by the scientist.

How Does CRISPR-Cas9 Work?

Guide RNA (gRNA)
1. A short RNA sequence that guides Cas9 to the specific DNA target.
Cas9 enzyme
1. Acts as molecular scissors, cutting the DNA at the target site.
DNA repair
1. The cell repairs the break, letting scientists disable a gene, insert a new gene, or correct a mutation.

Analogy

Think of CRISPR-Cas9 as GPS-guided scissors.
The gRNA is the GPS, directing Cas9 to the exact spot to cut.

Steps Of CRISPR-Cas9 Gene Editing

Design the gRNA to match the target DNA sequence.
Deliver the gRNA and Cas9 into the cell.
Target recognition and cutting:
1. The gRNA binds to the target DNA.

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Lorem ipsum dolor sit amet, consectetur adipiscing elit.
Sed do eiusmod tempor incididunt ut labore et dolore magna aliqua.
Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris.
Duis aute irure dolor in reprehenderit in voluptate velit esse cillum.

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CRISPR-Cas9 Edits DNA At A Chosen Site

CRISPR-Cas9 is a gene-editing tool adapted from a bacterial defense system.
Unlike a random mutation, it targets a specific DNA sequence chosen by the scientist.

How Does CRISPR-Cas9 Work?

Guide RNA (gRNA)
1. A short RNA sequence that guides Cas9 to the specific DNA target.
Cas9 enzyme
1. Acts as molecular scissors, cutting the DNA at the target site.
DNA repair
1. The cell repairs the break, letting scientists disable a gene, insert a new gene, or correct a mutation.

Analogy

Think of CRISPR-Cas9 as GPS-guided scissors.
The gRNA is the GPS, directing Cas9 to the exact spot to cut.

Steps Of CRISPR-Cas9 Gene Editing

Design the gRNA to match the target DNA sequence.
Deliver the gRNA and Cas9 into the cell.
Target recognition and cutting:
1. The gRNA binds to the target DNA.

Definition

Paywall

(on a website) an arrangement whereby access is restricted to users who have paid to subscribe to the site.

anim nostrud sit dolore minim proident quis fugiat velit et eiusmod nulla quis nulla mollit dolor sunt culpa aliqua

Duis aute irure dolor in reprehenderit

Note

Lorem ipsum dolor sit amet, consectetur adipiscing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam quis nostrud exercitation.

Excepteur sint occaecat cupidatat non proident

Tip

Lorem ipsum dolor sit amet, consectetur adipiscing elit.
Sed do eiusmod tempor incididunt ut labore et dolore magna aliqua.
Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris.
Duis aute irure dolor in reprehenderit in voluptate velit esse cillum.

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A - Unity and Diversity9 subtopics

B - Form and Function10 subtopics

C - Interaction and Interdependence9 subtopics

D - Continuity and Change12 subtopics

IB Biology Topic D1.3.9: Use of the CRISPR Sequences and the Enzyme Cas9 in Gene Editing Notes

CRISPR-Cas9 Edits DNA At A Chosen Site

How Does CRISPR-Cas9 Work?

Steps Of CRISPR-Cas9 Gene Editing

anim nostrud sit dolore minim proident quis fugiat velit et eiusmod nulla quis nulla mollit dolor sunt culpa aliqua

Duis aute irure dolor in reprehenderit

Excepteur sint occaecat cupidatat non proident

Unlock the rest of this chapter with a free account

CRISPR-Cas9 Edits DNA At A Chosen Site

How Does CRISPR-Cas9 Work?

Steps Of CRISPR-Cas9 Gene Editing

anim nostrud sit dolore minim proident quis fugiat velit et eiusmod nulla quis nulla mollit dolor sunt culpa aliqua

Duis aute irure dolor in reprehenderit

Excepteur sint occaecat cupidatat non proident

Unlock the rest of this chapter with a free account

A - Unity and Diversity9 subtopics

B - Form and Function10 subtopics

C - Interaction and Interdependence9 subtopics

D - Continuity and Change12 subtopics