Measuring Stomatal Density on Leaf Surfaces
Definition
Stomata
Stomata are small openings on the leaf surface that regulate gas exchange and water loss in plants
- Stomatal density refers to the number of stomata per unit area of a leaf surface.To determine this, the number of stomata in a known area must be counted using a microscope.
- Guard cells and stomata are microscopic, making them invisible to the naked eye but easily visible with proper tools.
Techniques for Measuring Stomatal Density
1. Peeling the Epidermis
- A section of the epidermis (outer leaf layer) is peeled off.
- Steps:
- Fold the leaf to break tissues apart from the lower epidermis.
- Carefully peel off the epidermis or tear the leaf diagonally to expose epidermal sections.
- Mount the epidermis in water on a microscope slide.
- Suitable Species: This method works well for plants like Commelina and Tradescantia.
Experiment with different plant species to refine this peeling technique
2. Using Nail Varnish
- Works best for smooth, non-hairy leaves.
- Steps:
