Chemistry IA Exemplar: How does the concentration of alcalase… | RevisionDojo

Loading document preview...

IB Chemistry HL Internal Assessment Example

How does the concentration of alcalase concentrate (0.00%, 0.50%, 1.00%, 1.50%, 2.00%) affect the average rate of 40.00 gdm-3 ovalbumin degradation after 5 minutes, measured using a Bradford protein assay and the absorbance at a wavelength of 595 nm with spectrophotometry?HL

Overall Score

21/24

Criteria A: Research Design

6/6

Criteria Strands

A.1Research question context

Excellent

A.2Methodological considerations

Good

A.3Methodology description

Good

Criteria Feedback

Your research question is focused, specific, and framed in a clear biochemical context.
You justify several important design choices with a preliminary investigation and repeated trials.
Your method is detailed enough that another reader could follow the main experimental sequence.

Key controls are not fully addressed, especially pH and whether the assay truly stops enzyme activity at the chosen endpoint.
The manipulated variable is only nominal because the active enzyme concentration or activity is not verified.
A few procedural details needed for full reproducibility are missing, such as exact calibration preparation and the delay between reagent addition and reading.

Criteria B: Data Analysis

5/6

Criteria Strands

B.1Communication of data recording and processing

Good

B.2Consideration of uncertainties

Moderate

B.3Data processing quality

Moderate

Criteria Feedback

You present the raw data clearly and in an organised way before moving into processed results.
Your use of means, standard deviations, calibration, and graphical presentation makes the processing easy to follow.
You show awareness of uncertainty through repeated measurements, error bars, and discussion of variability.

Some uncertainty expressions are not consistently defined across all concentrations, especially at the endpoints.
The calibration and conversion process is not fully robust because the assay response to hydrolysed products is not established.
A few processed values, such as the control uncertainty and the baseline concentration, raise concerns about consistency and chemical validity.

Criteria C: Conclusion

6/6

Criteria Strands

C.1Conclusion relevance and support

Moderate

C.2Scientific context comparison

Excellent

Criteria Feedback

Lorem ipsum dolor sit amet, consectetur adipiscing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua.
Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat.

Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur.
Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.

Criteria D: Evaluation

4/6

Criteria Strands

D.1Methodological weaknesses

Excellent

D.2Suggested improvements

Excellent

Criteria Feedback

Lorem ipsum dolor sit amet, consectetur adipiscing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua.
Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat.

Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur.
Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.

Loading scores...

Overall Score

21/24

Criteria A: Research Design

6/6

Criteria Strands

A.1Research question context

Excellent

A.2Methodological considerations

Good

A.3Methodology description

Good

Criteria Feedback

Your research question is focused, specific, and framed in a clear biochemical context.
You justify several important design choices with a preliminary investigation and repeated trials.
Your method is detailed enough that another reader could follow the main experimental sequence.

Key controls are not fully addressed, especially pH and whether the assay truly stops enzyme activity at the chosen endpoint.
The manipulated variable is only nominal because the active enzyme concentration or activity is not verified.
A few procedural details needed for full reproducibility are missing, such as exact calibration preparation and the delay between reagent addition and reading.

Criteria B: Data Analysis

5/6

Criteria Strands

B.1Communication of data recording and processing

Good

B.2Consideration of uncertainties

Moderate

B.3Data processing quality

Moderate

Criteria Feedback

You present the raw data clearly and in an organised way before moving into processed results.
Your use of means, standard deviations, calibration, and graphical presentation makes the processing easy to follow.
You show awareness of uncertainty through repeated measurements, error bars, and discussion of variability.

Some uncertainty expressions are not consistently defined across all concentrations, especially at the endpoints.
The calibration and conversion process is not fully robust because the assay response to hydrolysed products is not established.
A few processed values, such as the control uncertainty and the baseline concentration, raise concerns about consistency and chemical validity.

Criteria C: Conclusion

6/6

Criteria Strands

C.1Conclusion relevance and support

Moderate

C.2Scientific context comparison

Excellent

Criteria Feedback

Lorem ipsum dolor sit amet, consectetur adipiscing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua.
Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat.

Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur.
Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.

Criteria D: Evaluation

4/6

Criteria Strands

D.1Methodological weaknesses

Excellent

D.2Suggested improvements

Excellent

Criteria Feedback

Lorem ipsum dolor sit amet, consectetur adipiscing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua.
Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat.

Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur.
Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.