Investigating the effects of Iron (II) sulphate and copper (II) sulphate solution concentration on the decomposition of hydrogen peroxide into water and oxygen from catalase found in Ovis aries liver solution.
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Overall Score: 19/24
IB Grade: 6
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19/24
0
12
24
5.1·suggestion
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Units for inhibitor concentration mix “g/cm³” and “%”; clarify units consistently (e.g. % w/v) to avoid confusion.
5.2·weakness
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Qualitative observations document effervescence and colour changes but are not referenced in later data interpretation.
Criteria A: Research Design
5/6
0
3
6
Criteria Strands
Excellent
Research question context
Excellent
Methodological considerations
Good
Methodology description
Criteria Feedback
Research question is situated in a specific biological context with precise reagents, concentrations, volumes and time frames
Methodological considerations are well explained, linking controlled variables to their impact on reaction rate and addressing safety/ethical issues
Step-by-step methodology provides sufficient detail for reproducibility, including equipment, volumes and incubation times
Background omits discussion of industrial or medical relevance, limiting broader context
Controlled‐variables table has duplicated volume entries (CV4 and CV7) creating minor ambiguity
The method description lacks specification of bung material and leak‐test procedure
Risk mitigation for handling CuSO₄ is incomplete, omitting PPE and procedural controls
Criteria B: Data Analysis
5/6
0
3
6
Criteria Strands
Excellent
Communication of data recording and processing
Moderate
Consideration of uncertainties
Good
Data processing quality
Criteria Feedback
Data recording and processing are communicated clearly and precisely, with well‐labelled tables, units, equipment uncertainties, and error bars
Statistical analyses—including means, standard deviations, Pearson correlation, and Mann–Whitney U tests—are correctly executed and interpreted
Graphical presentation is polished: axes labels, significant figures and descriptive title enhance clarity
Instrument uncertainties are recorded but not propagated into processed data, weakening the uncertainty analysis
Standard deviation is conflated with instrument uncertainty rather than treated separately
Some conceptual confusion exists around the interpretation of r² and justification for omitting the regression line
Rationale for statistical test choices (e.g., Mann–Whitney) is not fully articulated
Criteria C: Conclusion
4/6
0
3
6
Criteria Strands
Good
Conclusion relevance and consistency
Good
Scientific context comparison
Criteria Feedback
Conclusion correctly identifies and quantifies the inverse relationship between inhibitor concentration and enzyme activity
There is consistency between the stated conclusion and the statistical analysis, with anomalies acknowledged
Relevant comparisons to scientific literature on catalase and metal‐ion interactions are made
Misinterpretation of p = 0.053 one‐tailed without addressing two‐tailed context or confidence intervals
Limited critical discussion of quantitative discrepancies between experimental results and published data
The justification, while consistent, lacks full rigor in statistical interpretation
Criteria D: Evaluation
5/6
0
3
6
Criteria Strands
Excellent
Methodological weaknesses
Excellent
Improvements
Criteria Feedback
Specific methodological weaknesses are identified and their relative impacts are qualitatively explained
Realistic improvements directly address each identified limitation, showing clear linkage between issue and remedy
The evaluative narrative ranks the significance of each limitation, demonstrating depth of reflection
Quantitative estimates of the impact of limitations (e.g., percent error) are not provided
Some proposed extensions (e.g., temperature variation) lack detailed experimental parameters such as specific temperature points and buffer systems
Calibration procedures for suggested equipment (e.g., pressure sensor) are not described
Biology IA Exemplar: Iron(II) Sulphate and Copper(II) Sulphate on Catalase Decomposition | RevisionDojo