Biology IA Exemplar: Ethanol Concentration and Alpha-Amylase Starch Hydrolysis | RevisionDojo
IB Biology SL Internal Assessment Exemplar
How does increasing the concentration of ethanol (0%, 5%, 10%, 15%, 20%,
25%) affect the rate of hydrolysis of starch by alpha-amylase measured as the change in absorbance
units per minute (AU/min) over a 5 minute incubation period?
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Overall Score: 10/24
IB Grade: 3
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10/24
0
12
24
5.1·strength
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The materials list is comprehensive, covering all apparatus and reagents needed for the investigation.
5.2·suggestion
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The risk assessment is thorough; consider adding details on ethanol waste disposal procedures to fully address laboratory safety.
Criteria A: Research Design
4/6
0
3
6
Criteria Strands
Excellent
Research question context
Excellent
Methodological considerations
Good
Methodology description
Criteria Feedback
Research question situated in a clear, specific biochemical context with relevant literature justification
Comprehensive identification and control of variables; justification for replicate number and concentration increments
Thoughtful preliminary trial demonstrating planning for plateau determination
Method description contains ambiguities (e.g. mixing order of iodine, ethanol, starch)
Cuvette fill given as “¾ full” without specifying path length or exact volume
Controlled‐variable table omits full specification for starch solution volume
Criteria B: Data Analysis
2/6
0
3
6
Criteria Strands
Good
Communication of data recording and processing
Poor
Consideration of uncertainties
Moderate
Data processing quality
Criteria Feedback
Raw and processed data are tabulated clearly and graphs include appropriate titles, labels, and error bars
A one-way ANOVA is attempted, showing engagement with relevant statistical tools
Absorbance units are missing and table headings are cluttered
Instrument uncertainties and their propagation are omitted, error bars represent only SD
Computational slips in standard deviation and ANOVA calculations introduce inaccuracies
Criteria C: Conclusion
2/6
0
3
6
Criteria Strands
Moderate
Conclusion relevance and consistency
Moderate
Scientific context comparison
Criteria Feedback
Conclusion restates key trend and links findings to the research question
Brief comparison to accepted scientific context highlights mechanism considerations
Conclusion is not fully consistent with the analytical data (no reaction‐rate calculations)
Discussion lacks quantitative comparison to literature values for deeper justification
Criteria D: Evaluation
2/6
0
3
6
Criteria Strands
Moderate
Methodological weaknesses
Moderate
Improvements
Criteria Feedback
Specific methodological weaknesses (e.g. pH control, particle deposition) are identified
Realistic improvements (buffers, increased trials, better instruments) are proposed
The relative impact of each weakness on validity is not explained
Improvements are described without discussing how they would quantitatively enhance reliability