Biology IA Exemplar: Starch Concentration and Amylase Activity | RevisionDojo
IB Biology SL Internal Assessment Exemplar
How do different concentrations ( 0%, 2%, 4%, 6% and 8%) of starch alter the activity of amylase in the hydrolysis of starch measured through visual color changes and changes in light transmission(%)?
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Overall Score: 14/24
IB Grade: 5
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Criteria A: Research Design
5/6
0
3
6
Criteria Strands
Excellent
Research question context
Excellent
Methodological considerations
Good
Methodology description
Criteria Feedback
Research question precisely specifies independent and dependent variables within a clear biochemical context.
Control variables are comprehensively listed with clear methods and rationales.
Methodological considerations are well justified by citing prior research on optimal starch concentration.
Typographical errors in “Repeat steps 2–85 times” create ambiguity in repeat count.
Iodine–colorimeter procedure lacks explicit timing and measurement-interval details.
Bundled visual and quantitative measures of the dependent variable are not clearly delineated.
Criteria B: Data Analysis
2/6
0
3
6
Criteria Strands
Good
Communication of data recording and processing
Poor
Consideration of uncertainties
Moderate
Data processing quality
Criteria Feedback
Raw data tables include units, uncertainties, and descriptive titles.
Worked example for the 6% concentration calculation clearly demonstrates processing steps.
Statistical analysis (unpaired t-test) with p-value and confidence interval is appropriately presented.
Uncertainties listed are not propagated through calculations, and error bars do not derive from uncertainty analysis.
Graph axes are mislabeled (‘light absorbance’ vs. ‘transmission’) and omit units and error bars.
Negative reaction rate at 4% is noted but not analyzed or explained.
Criteria C: Conclusion
4/6
0
3
6
Criteria Strands
Excellent
Conclusion relevance and consistency
Good
Scientific context comparison
Criteria Feedback
Conclusion clearly restates the aim and identifies 6% starch as yielding the highest activity, supported by mean rates and statistical significance.
Comparison to Gebreyohannes (2015) situates results within accepted scientific context and justifies methodological discrepancies.
Brief treatment of the anomalous negative rate at 4%; kinetic or measurement-error analysis is needed to fully justify consistency.
Discussion of differences in pH and temperature effects is sound but could be more analytically detailed.
Criteria D: Evaluation
3/6
0
3
6
Criteria Strands
Moderate
Methodological weaknesses
Moderate
Improvements
Criteria Feedback
Specific methodological weaknesses (temperature drift, volumetric inaccuracies, qualitative iodine endpoint) are identified.
Improvements (use volumetric pipette, relocate colorimeter, extend duration) are realistic and directly address identified issues.
Acknowledgement of limitations in the qualitative iodine test demonstrates critical evaluation of reliability concerns.
No quantitative estimation of how temperature drift or volumetric error impacts results.
Suggested improvements are described but lack detailed explanation of how they would enhance validity.
Innovative proposals (e.g., testing mango amylase) need clearer justification in relation to the research question.